{"id":352438,"date":"2025-07-30T12:17:19","date_gmt":"2025-07-30T11:17:19","guid":{"rendered":"https:\/\/zakrasnejsivimperk.cz\/?p=352438"},"modified":"2025-08-02T19:46:19","modified_gmt":"2025-08-02T18:46:19","slug":"aktuality-vyzkum-odhalil-detaily-fungovani-enzymu-ktery-reguluje-sodik-v-tele","status":"publish","type":"post","link":"https:\/\/zakrasnejsivimperk.cz\/?p=352438","title":{"rendered":"AKTUALITY: V\u00fdzkum odhalil detaily fungov\u00e1n\u00ed enzymu, kter\u00fd reguluje sod\u00edk v t\u011ble"},"content":{"rendered":"

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Mechanismus regulace enzymu E3 ubikvitin ligasy Nedd4-2 poprv\u00e9 podrobn\u011b popsal t\u00fdm Veroniky Ob\u0161ilov\u00e9 z\u00a0Fyziologick\u00e9ho \u00fastavu AV \u010cR a Tom\u00e1\u0161e Ob\u0161ila z\u00a0katedry fyzik\u00e1ln\u00ed a\u00a0makromolekul\u00e1rn\u00ed chemie P\u0159\u00edrodov\u011bdeck\u00e9 fakulty Univerzity Karlovy. Studie vy\u0161la v\u00a0presti\u017en\u00edm \u010dasopise Nature Communications.<\/h6>\n

Sod\u00edk je \u017eivotn\u011b d\u016fle\u017eit\u00fd prvek, ale jeho nadbytek t\u011blu \u0161kod\u00ed \u2013 zp\u016fsobuje nap\u0159\u00edklad zadr\u017eov\u00e1n\u00ed vody v\u00a0organismu, otoky nebo zv\u00fd\u0161en\u00fd objem krve. Z\u00e1sadn\u00ed roli v regulaci hladiny sod\u00edku hraje enzym Nedd4-2: kontroluje odstra\u0148ov\u00e1n\u00ed sod\u00edkov\u00fdch kan\u00e1l\u016f z\u00a0bun\u011b\u010dn\u00fdch membr\u00e1n. To je kl\u00ed\u010dov\u00e9 pro regulaci krevn\u00edho tlaku a osmotick\u00e9 rovnov\u00e1hy, tedy vyv\u00e1\u017een\u00e9 koncentrace l\u00e1tek pohybuj\u00edc\u00edch se mezi bu\u0148kami a okol\u00edm. Porucha regulace toti\u017e souvis\u00ed s\u00a0onemocn\u011bn\u00edmi, jako jsou vysok\u00fd krevn\u00ed tlak, choroby ledvin \u010di\u00a0n\u011bkter\u00e9 druhy rakoviny.<\/p>\n

V nov\u00e9 studii t\u00fdm z Fyziologick\u00e9ho \u00fastavu AV \u010cR a P\u0159\u00edrodov\u011bdeck\u00e9 fakulty Univerzity Karlovy poprv\u00e9 detailn\u011b popsal strukturu cel\u00e9ho enzymu Nedd4-2 a vysv\u011btlil mechanismus jeho regulace. \u201ePomoc\u00ed pokro\u010dil\u00fdch zobrazovac\u00edch a biochemick\u00fdch metod jsme zjistili, \u017ee funkce Nedd4-2 je blokov\u00e1na vz\u00e1jemn\u00fdmi interakcemi jeho dom\u00e9n, to je \u010d\u00e1st\u00ed enzymu se specifickou \u00falohou a strukturou,\u201c<\/em> \u0159\u00edk\u00e1 Veronika Ob\u0161ilov\u00e1 z\u00a0Fyziologick\u00e9ho \u00fastavu AV \u010cR, koresponduj\u00edc\u00ed autorka zve\u0159ejn\u011bn\u00e9 studie.<\/p>\n

Enzym podle n\u00ed z\u016fst\u00e1v\u00e1 neaktivn\u00ed, dokud se v p\u0159\u00edtomnosti v\u00e1pn\u00edkov\u00fdch iont\u016f nenav\u00e1\u017ee na bun\u011b\u010dnou membr\u00e1nu. To vede k uvoln\u011bn\u00ed mezi-dom\u00e9nov\u00fdch interakc\u00ed a k aktivaci enzymu Nedd4-2.<\/p>\n

Studie tak\u00e9 prok\u00e1zala, \u017ee na regulaci Nedd4-2 se d\u00e1le pod\u00edlej\u00ed i proteiny 14-3-3, kter\u00e9 reaguj\u00ed na hormon\u00e1ln\u00ed sign\u00e1ly a svoj\u00ed vazbou blokuj\u00ed jak enzymatickou aktivitu Nedd4-2, tak i jeho schopnost v\u00e1zat se na membr\u00e1nu.<\/p>\n

Odkaz na publikaci:<\/strong><\/p>\n

Masa Janosev, Dalibor Kosek, Andrej Tekel, Rohit Joshi, Karolina Honzejkova, Pavel Pohl, Tomas Obsil* & Veronika Obsilova*. Structural basis of ubiquitin ligase Nedd4-2 autoinhibition and regulation by calcium and 14-3-3 proteins. Nature Communications 16:4875 (2025). DOI: 10.1038\/s41467-025-60207-4. IF = 14.7<\/p>\n

(* shared corresponding authorship)<\/p>\n

https:\/\/www.nature.com\/articles\/s41467-025-60207-4<\/strong><\/a><\/p>\n

(tz-av\u010dr)<\/strong><\/p>\n

\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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