{"id":252630,"date":"2022-05-09T15:12:03","date_gmt":"2022-05-09T14:12:03","guid":{"rendered":"https:\/\/zakrasnejsivimperk.cz\/?p=252630"},"modified":"2022-05-28T19:27:58","modified_gmt":"2022-05-28T18:27:58","slug":"veda-a-priroda-vedci-objevili-unikatni-struktury-v-geneticke-informaci-viru-klistove-encefalitidy","status":"publish","type":"post","link":"https:\/\/zakrasnejsivimperk.cz\/?p=252630","title":{"rendered":"V\u011aDA A P\u0158\u00cdRODA: V\u011bdci objevili unik\u00e1tn\u00ed struktury v genetick\u00e9 informaci viru kl\u00ed\u0161\u0165ov\u00e9 encefalitidy"},"content":{"rendered":"

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Unik\u00e1tn\u00ed struktury, ozna\u010dovan\u00e9 jako guaninov\u00e9 kvadruplexy (G4), objevil t\u00fdm \u010desk\u00fdch v\u011bdc\u016f v genetick\u00e9 informaci viru kl\u00ed\u0161\u0165ov\u00e9 encefalitidy. Odborn\u00edci zjistili, \u017ee tyto struktury hraj\u00ed d\u016fle\u017eitou roli p\u0159i mno\u017een\u00ed viru a lze na n\u011b c\u00edlit p\u0159i hled\u00e1n\u00ed nov\u00fdch antivirotik. P\u0159i testov\u00e1n\u00ed potenci\u00e1ln\u00edch chemick\u00fdch l\u00e1tek, kter\u00e9 rozpozn\u00e1vaj\u00ed tyto G4 struktury, na\u0161li slibn\u00e9 molekuly s vysok\u00fdm antivirov\u00fdm \u00fa\u010dinkem. Tyto l\u00e1tky tak mohou v budoucnu roz\u0161\u00ed\u0159it reperto\u00e1r mo\u017en\u00fdch l\u00e9\u010div na tuto infekci.<\/b><\/h6>\n

\"\"Virus kl\u00ed\u0161\u0165ov\u00e9 encefalitidy zp\u016fsobuje z\u00e1va\u017en\u00e9 onemocn\u011bn\u00ed nervov\u00e9 soustavy \u010dlov\u011bka. V posledn\u00edch letech nav\u00edc celosv\u011btov\u011b vzr\u016fst\u00e1 po\u010det p\u0159\u00edpad\u016f i p\u0159esto, \u017ee je k dispozici \u00fa\u010dinn\u00e9 o\u010dkov\u00e1n\u00ed. V p\u0159\u00edpad\u011b n\u00e1kazy v\u0161ak zat\u00edm nejsou dostupn\u00e1 \u017e\u00e1dn\u00e1 vhodn\u00e1 antivirotika, proto jsou pacienti odk\u00e1z\u00e1ni pouze na podp\u016frnou l\u00e9\u010dbu.<\/p>\n

Na v\u00fdzkumu guaninov\u00fdch kvadruplex\u016f u viru kl\u00ed\u0161\u0165ov\u00e9 encefalitidy spolupracovali biofyzikov\u00e9 a virologov\u00e9 z Biofyzik\u00e1ln\u00edho \u00fastavu AV \u010cR, V\u00fdzkumn\u00e9ho \u00fastavu veterin\u00e1rn\u00edho l\u00e9ka\u0159stv\u00ed, Parazitologick\u00e9ho \u00fastavu Biologick\u00e9ho centra AV \u010cR, \u00dastavu organick\u00e9 chemie a biochemie AV \u010cR, P\u0159\u00edrodov\u011bdeck\u00e9 fakulty Masarykovy univerzity a \u010cesk\u00e9 zem\u011bd\u011blsk\u00e9 univerzity v Praze. \u201eStruktura guaninov\u00fdch kvadruplex\u016f byla zn\u00e1m\u00e1 u\u017e u n\u011bkter\u00fdch jin\u00fdch vir\u016f. U viru kl\u00ed\u0161\u0165ov\u00e9 encefalitidy ale je\u0161t\u011b nik\u00fdm popsan\u00e1 nebyla. Proto jsme se rozhodli prozkoumat genom tohoto viru a zjistit, zda se tam guaninov\u00e9 kvadruplexy vyskytuj\u00ed a jakou maj\u00ed strukturu. Zam\u011b\u0159ili jsme se i na to, jak mohou ovliv\u0148ovat virovou infekci v hostitelsk\u00fdch bu\u0148k\u00e1ch,\u201c<\/em> \u0159\u00edk\u00e1 hlavn\u00ed autor studie Ji\u0159\u00ed Holoubek z Parazitologick\u00e9ho \u00fastavu Biologick\u00e9ho centra AV \u010cR a V\u00fdzkumn\u00e9ho \u00fastavu veterin\u00e1rn\u00edho l\u00e9ka\u0159stv\u00ed a z\u00e1rove\u0148 doktorsk\u00fd student na P\u0159\u00edrodov\u011bdeck\u00e9 fakult\u011b Masarykovy univerzity, kter\u00fd svou diserta\u010dn\u00ed pr\u00e1ci vypracov\u00e1v\u00e1 pod veden\u00edm virolog\u016f Daniela R\u016f\u017eka a Lu\u010fka Eyera.<\/p>\n

Nalezen\u00ed guaninov\u00fdch kvadruplex\u016f v genomu viru kl\u00ed\u0161\u0165ov\u00e9 encefalitidy prob\u00edhalo v \u00fazk\u00e9 spolupr\u00e1ci s t\u00fdmem biofyzik\u016f. \u201ePomoc\u00ed bioinformatick\u00fdch metod jsme vytipovali, na kter\u00fdch m\u00edstech genomu se tyto struktury vytv\u00e1\u0159ej\u00ed a charakterizovali jsme je. Na molekul\u00e1rn\u00ed \u00farovni in vitro, tedy ve zkumavce, jsme tak\u00e9 sledovali aktivitu molekul, kter\u00e9 jsou schopn\u00e9 guaninov\u00e9 kvadruplexy rozpozn\u00e1vat,\u201c<\/em> \u0159\u00edk\u00e1 Kl\u00e1ra Bedn\u00e1\u0159ov\u00e1 z Biofyzik\u00e1ln\u00edho \u00fastavu AV \u010cR, druh\u00e1 hlavn\u00ed autorka studie. Biologov\u00e9 pak jejich v\u00fdsledky ov\u011b\u0159ovali p\u0159i re\u00e1ln\u00e9 infekci v bu\u0148k\u00e1ch napaden\u00fdch \u017eiv\u00fdm virem.<\/p>\n

Uk\u00e1zalo se, \u017ee guaninov\u00e9 kvadruplexy hraj\u00ed kl\u00ed\u010dovou roli p\u0159i mno\u017een\u00ed viru v hostitelsk\u00e9 bu\u0148ce, \u017ee jejich struktura v\u00fdznamn\u011b ovliv\u0148uje \u017eivotaschopnost a biologick\u00e9 vlastnosti viru a \u017ee je mo\u017en\u00e9 na n\u011b c\u00edlit chemick\u00fdmi l\u00e1tkami, kter\u00e9 zabr\u00e1n\u00ed virov\u00e9 infekci. V\u011bdci identifikovali n\u011bkolik molekul, kter\u00e9 vykazovaly antivirov\u00fd \u00fa\u010dinek a v budoucnu by se tak mohly st\u00e1t mo\u017en\u00fdmi kandid\u00e1ty pro v\u00fdvoj nov\u00e9 generace protivirov\u00fdch l\u00e9\u010div.<\/p>\n

Studie vznikla v r\u00e1mci \u0159e\u0161en\u00ed projektu financovan\u00e9ho Grantovou agenturou \u010cesk\u00e9 republiky (20-20229S), jeho\u017e garanty jsou Daniel Ren\u010diuk (Biofyzik\u00e1ln\u00ed \u00fastav AV \u010cR) a Lud\u011bk Eyer (V\u00fdzkumn\u00fd \u00fastav veterin\u00e1rn\u00edho l\u00e9ka\u0159stv\u00ed). V\u00fdsledky studie byly publikov\u00e1ny v aktu\u00e1ln\u00edm \u010d\u00edsle presti\u017en\u00edho v\u011bdeck\u00e9ho \u010dasopisu\u00a0Nucleic Acids Research<\/a><\/strong>.<\/p>\n

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Text a foto: (tzav\u010dr)<\/strong><\/p>\n

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  Unik\u00e1tn\u00ed <\/p>\n","protected":false},"author":2,"featured_media":252632,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"gallery","meta":{"footnotes":""},"categories":[24,174],"tags":[],"jetpack_featured_media_url":"https:\/\/zakrasnejsivimperk.cz\/wp-content\/uploads\/2022\/04\/23\/kl\u00ed\u0161t\u011b-Foto-Jan-Erhart-BC-AV-CR.jpg","_links":{"self":[{"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=\/wp\/v2\/posts\/252630"}],"collection":[{"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=252630"}],"version-history":[{"count":3,"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=\/wp\/v2\/posts\/252630\/revisions"}],"predecessor-version":[{"id":253876,"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=\/wp\/v2\/posts\/252630\/revisions\/253876"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=\/wp\/v2\/media\/252632"}],"wp:attachment":[{"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=252630"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=252630"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/zakrasnejsivimperk.cz\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=252630"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}